Phospho-VASP (Ser239) Antibody

About the Target

Phospho-VASP (Vasodilator-stimulated phosphoprotein) (Ser239) is a ubiquitously expressed actin-binding protein of the conserved Ena/VASP family that regulates cell shape, polarity, adhesion, migration, and invasion by scaffolding regulatory proteins with growing actin filaments at dynamic membrane regions such as lamellipodia, filopodia, invadopodia, focal adhesions, and cell-cell junctions. Structurally, it contains an N-terminal EVH1 domain for binding proline-rich partners (e. g., vinculin, zyxin), a central proline-rich region that interacts with SH3/WW domain proteins and profilin-G-actin, and a C-terminal EVH2 domain that binds G- and F-actin and mediates oligomerization to promote filament bundling. Depending on the literature source, VASP may also be discussed as Phospho-VASP (Ser239) and Vasodilator-stimulated phosphoprotein.

Reported cellular context includes cell junction, cell membrane, cell projection, and cytoplasm, which can matter when signal is compared across treatments or changing cell states. Following VASP across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

VASP is commonly interpreted in the context of cancer research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cell junction, cell membrane, and cell projection, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between cell junction, cell membrane, and cell projection across matched conditions
• changes associated with proliferative state, oncogenic signaling, or treatment response
• differences between total target abundance and site-specific regulation when modified forms are compared
• co-patterning with orthogonal markers and control conditions that clarify pathway state

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for VASP. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in VASP reflect biology rather than handling. When interpreting VASP, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep VASP trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.