Phospho-Tuberin/TSC2 (Ser1387) Antibody

About the Target

Phospho-Tuberin/TSC2 (Ser1387) refers to the phosphorylation of serine 1387 on the tuberin protein (TSC2), encoded by the TSC2 gene. This phosphorylation is crucial for regulating the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway. Located in the C-terminal region of TSC2, Ser1387 is phosphorylated by AMP-activated protein kinase (AMPK), which activates TSC2 and enhances its role as a GTPase-activating protein (GAP) for RHEB, thereby inhibiting mTORC1. Depending on the literature source, TSC2 may also be discussed as Phospho-Tuberin/TSC2 (Ser1387) and Phospho Tuberin (Ser 1387).

Reported cellular context includes cytoplasm, lysosome, and membrane, which can matter when signal is compared across treatments or changing cell states. Following TSC2 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

TSC2 is commonly interpreted in the context of cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cytoplasm, lysosome, and membrane, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between cytoplasm, lysosome, and membrane across matched conditions
• signal-dependent shifts after ligand, inhibitor, or growth-factor perturbation
• differences between total target abundance and site-specific regulation when modified forms are compared
• co-patterning with orthogonal markers and control conditions that clarify pathway state

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for TSC2. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in TSC2 reflect biology rather than handling. When interpreting TSC2, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep TSC2 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.