Trpv3 Antibody - Western Blot Validated
BiCell Scientific
SKU:11033-WB-50UL
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Protein Function
When control selection matters, this antibody helps you evaluate Trpv3 in a structured study. Trpv3 is a membrane protein linked to solute movement and gradient maintenance. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.
Tracking Trpv3 can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Experimental Applications
Many transport proteins cycle through endosomal pathways, making localization a key readout alongside abundance. Time course design can be decisive when regulation is transient or compartment dependent.
Many workflows benefit from tracking both signal intensity and compartment distribution, especially when the target participates in multi-protein assemblies.
Common experimental questions that benefit from target tracking include:
- surface versus intracellular pool distribution
- patterns consistent with apical or basolateral sorting
- relative abundance across engineered constructs
Workflow Perspective
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Predefine acceptance criteria for controls before collecting the full dataset.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Trpv3 patterns can be compared across experiments without guesswork.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Trpv3 patterns can be compared across experiments without guesswork.
The denaturing-format format fits designs where Trpv3 is evaluated with deliberate controls and careful comparisons.
- Targets:
- TRPV3
- Research Area:
- Cell Signaling • Neuroscience
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (15-aa) derived from the C-terminal region of mouse Trpv3 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q8NET8
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 86.7% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C