Trpc6 (C-Terminus) Antibody - Western Blot Validated
BiCell Scientific
SKU:11016-WB-50UL
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Protein Function
Build reproducible readouts by using this antibody to characterize Trpc6 C-Terminus across matched sample sets. Trpc6 C-Terminus is a channel or transporter associated with regulated flux across cellular membranes. Use matched handling so differences reflect biology rather than processing.
Following Trpc6 C-Terminus across perturbations can help separate expression effects from compartment shifts.
Experimental Applications
Transport targets are commonly studied under stimulation conditions that alter membrane potential, osmolarity, or nutrient availability. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
In practice, target tracking can support localization mapping, relative quantification, and interaction-focused experiments without locking you into a single readout strategy.
Common experimental questions that benefit from target tracking include:
- surface versus intracellular pool distribution
- stimulus-dependent trafficking and turnover
- patterns consistent with apical or basolateral sorting
- relative abundance across engineered constructs
Workflow Perspective
This denaturing-format version is geared toward targets presented after reduction and denaturation, where epitope exposure can shift from native conformations. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Document normalization choices so collaborators can reproduce the same interpretation.
If your study spans multiple model systems, it can help to define a shared reference condition for Trpc6 C-Terminus so cross-sample contrasts stay meaningful.
With consistent handling, the denaturing-format format can make Trpc6 C-Terminus comparisons easier to reproduce across runs.
- Targets:
- TRPC6
- Research Area:
- Cell Signaling • Neuroscience
- Application:
- WB
- Reactivity:
- Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (18-aa) derived from the C-terminal cytoplasmic region of mouse Trpc6 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9Y210
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 72.2% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C