Tetraspanin-25 (TSPAN25 or CD53) Antibody - Western Blot Validated

Biological Background

When you need to characterize Tetraspanin-25 (CD53) with attention to cellular organization, this antibody provides a focused option. Tetraspanin-25 is a matrix-interaction protein often examined in migration and morphogenesis models. Its behavior can reflect changes in differentiation state, stress, or remodeling of cellular architecture.

In some datasets and protocols, it is referenced by the gene symbol CD53.

Use in the Laboratory

Comparative experiments can separate expression differences from changes in adhesion structure abundance. Because regulation can be multi-layered, combining abundance and localization information can sharpen conclusions.

Interpreting changes can benefit from separating expression differences from redistribution, particularly when the target cycles between compartments.

To keep datasets interpretable, labs often document:

• effects of adhesion inhibitors or matrix composition
• focal adhesion versus diffuse membrane distribution
• co-variation with cytoskeletal and scaffold partners

Version Notes

This denaturing-format version is geared toward targets presented after reduction and denaturation, where epitope exposure can shift from native conformations. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. Small differences in preparation can reshape apparent localization, so interpret results in context.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Tetraspanin-25 patterns can be compared across experiments without guesswork.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Tetraspanin-25 patterns can be compared across experiments without guesswork.

The denaturing-format version supports deeper biological narratives around Tetraspanin-25 (CD53) without overcomplicating setup.