Tetraspanin-20 (TSPAN20 or Uroplakin-1b or UPK1B or UPK1) Antibody - FFPE Validated

About the Target

Plan your comparisons around Tetraspanin-20 (UPK1) and use this antibody to characterize state-dependent differences. Tetraspanin-20 is an adhesion-related target that can shift with substrate composition and mechanical cues. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

Monitoring Tetraspanin-20 alongside context markers can improve interpretation when phenotypes are subtle.

Research Context

Perturbations that disrupt integrin engagement or actomyosin tension can reshape localization and complex assembly. Time course design can be decisive when regulation is transient or compartment dependent.

Consider these angles when interpreting target-level changes:

• focal adhesion versus diffuse membrane distribution
• remodeling after changes in substrate or mechanical stress
• co-variation with cytoskeletal and scaffold partners

Variant Considerations

In sectioned tissue workflows, interpretation often depends on morphology; this format supports that style of readout. This matters because epitope exposure and background can vary with preparation and extraction steps. Include controls that separate true target shifts from matrix or preparation effects.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Tetraspanin-20 patterns can be compared across experiments without guesswork.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Tetraspanin-20 patterns can be compared across experiments without guesswork.

Use the fixed-section option to keep Tetraspanin-20 (UPK1) analysis aligned with planned contrasts and endpoints.