Tetraspanin-12 (TSPAN12) Antibody
BiCell Scientific
SKU:10212-50UL
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Protein Function
This antibody is suited for researchers who want to assess Tetraspanin-12 (TSPAN12) and relate it to cell-state biology. Tetraspanin-12 is a matrix-interaction protein often examined in migration and morphogenesis models. Its behavior can reflect changes in differentiation state, stress, or remodeling of cellular architecture.
In some datasets and protocols, it is referenced by the gene symbol TSPAN12.
Experimental Applications
Perturbations that disrupt integrin engagement or actomyosin tension can reshape localization and complex assembly. Because regulation can be multi-layered, combining abundance and localization information can sharpen conclusions.
In practice, target tracking can support localization mapping, relative quantification, and interaction-focused experiments without locking you into a single readout strategy.
Common experimental questions that benefit from target tracking include:
- effects of adhesion inhibitors or matrix composition
- focal adhesion versus diffuse membrane distribution
- co-variation with cytoskeletal and scaffold partners
Workflow Perspective
When assay conditions are still being tuned, a regular-format antibody supports stepwise adjustment of buffers and incubation steps. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series.
For challenging targets, pairing Tetraspanin-12 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so Tetraspanin-12 patterns can be compared across experiments without guesswork.
The regular format version supports deeper biological narratives around Tetraspanin-12 (TSPAN12) without overcomplicating setup.
- Targets:
- TSPAN12
- Research Area:
- Developmental Biology
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (14-aa) derived from the C-terminal cytoplasmic region of human Tetraspanin-12 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- O95859
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C