SMARCB1/BAF47 Antibody

About the Target

SMARCB1/BAF47 is a target of interest in many antibody-based workflows. SMARCB1, also known as SNF5, BAF47, is a crucial member of the SWI/SNF (SWItch/Sucrose Non-Fermentable) chromatin-remodeling multiprotein complex. This ubiquitously expressed nuclear protein, encoded by the SMARCB1 gene on chromosome 22 (22q11. 23), is involved in recruiting the SWI/SNF complex to the core promoter of rRNA genes via its interaction with PIH1 domain-containing protein 1 (PIH1) and histone H4, thus regulating chromatin remodelling. Depending on the literature source, SMARCB1/BAF47 may also be discussed as SMARCB1/BAF47 and BAF47.

Reported cellular context includes nucleus, which can matter when signal is compared across treatments or changing cell states. Following SMARCB1/BAF47 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

SMARCB1/BAF47 is commonly interpreted in the context of cancer and epigenetics research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans nucleus, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• signal enrichment within nucleus relative to the broader cellular background
• changes associated with proliferative state, oncogenic signaling, or treatment response
• links between target behavior and transcriptional or chromatin-state changes
• co-patterning with orthogonal markers and control conditions that clarify pathway state

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for SMARCB1/BAF47. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in SMARCB1/BAF47 reflect biology rather than handling. When interpreting SMARCB1/BAF47, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep SMARCB1/BAF47 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.