SLC12A8 (CCC9) Antibody
BiCell Scientific
SKU:20308-50UL
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Biological Background
For consistent comparison across experiments, use this antibody to characterize SLC12A8 (CCC9) with a repeatable workflow. SLC12A8 is a trafficking-sensitive transporter that can shift between intracellular pools and the plasma membrane. Use matched handling so differences reflect biology rather than processing.
In some datasets and protocols, it is referenced by the gene symbol CCC9.
Use in the Laboratory
Modulators that affect trafficking, phosphorylation, or ligand binding can change apparent distribution and signal intensity. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
Interpreting changes can benefit from separating expression differences from redistribution, particularly when the target cycles between compartments.
To keep datasets interpretable, labs often document:
- surface versus intracellular pool distribution
- patterns consistent with apical or basolateral sorting
- stimulus-dependent trafficking and turnover
- relative abundance across engineered constructs
Version Notes
For broad method development, the regular format can help you benchmark target behavior before narrowing to a specific readout style. If your study compares multiple conditions, keep acquisition and processing settings stable to avoid confounding. A brief pilot run can help set exposure ranges and reduce reruns later.
When interpreting SLC12A8, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
With consistent handling, the regular format format can make SLC12A8 (CCC9) comparisons easier to reproduce across runs.
- Targets:
- SLC12A8
- Research Area:
- Metabolism
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (14-aa) derived from the C-terminal cytoplasmic region of mouse SLC12A8 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- A0AV02
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 78.6% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C