PAX2 Antibody - Western Blot Validated
BiCell Scientific
SKU:51232-WB-50UL
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Protein Function
When control selection matters, this antibody helps you investigate PAX2 in a structured study. PAX2 is a molecular target often examined to connect phenotype changes with underlying protein regulation. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.
Tracking PAX2 can clarify whether changes reflect regulation, redistribution, or altered complex assembly.
Experimental Applications
Temporal sampling and matched controls can improve interpretation when expression is dynamic. Time course design can be decisive when regulation is transient or compartment dependent.
In practice, target tracking can support localization mapping, relative quantification, and interaction-focused experiments without locking you into a single readout strategy. Developmental projects may follow this target across stage-dependent remodeling and tissue patterning.
Common experimental questions that benefit from target tracking include:
- cross-sample normalization strategies
- interpretation alongside orthogonal readouts
- subcellular compartment mapping
Workflow Perspective
This denaturing-format version is geared toward targets presented after reduction and denaturation, where epitope exposure can shift from native conformations. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Predefine acceptance criteria for controls before collecting the full dataset.
For challenging targets, pairing PAX2 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
The denaturing-format format fits designs where PAX2 is evaluated with deliberate controls and careful comparisons.
- Targets:
- PAX2
- Research Area:
- Developmental Biology
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the internal region of human PAX2 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q02962
- Homology:
- Synthetic peptide sequence is identical to mouse or rat sequence
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C