Phospho-NDRG1 (Thr346) Antibody

About the Target

N-myc downstream-regulated gene 1 (NDRG1), also known as Cap43, Drg1, RTP/rit42, and Proxy-1, is a member of the NDRG family, which comprises four members (NDRG1-4) involved in growth, differentiation, and cell survival. NDRG1 is widely expressed and highly responsive to various stress signals such as DNA damage, hypoxia, and elevated levels of nickel and calcium. Depending on the literature source, NDRG1 may also be discussed as Phospho-NDRG1 (Thr346).

Reported cellular context includes cell membrane, cytoplasm, cytoskeleton, and membrane, which can matter when signal is compared across treatments or changing cell states. Following NDRG1 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

NDRG1 is commonly interpreted in the context of cancer, developmental biology, and cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cell membrane, cytoplasm, and cytoskeleton, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between cell membrane, cytoplasm, and cytoskeleton across matched conditions
• changes associated with proliferative state, oncogenic signaling, or treatment response
• stage-dependent patterns during differentiation, morphogenesis, or lineage commitment
• signal-dependent shifts after ligand, inhibitor, or growth-factor perturbation

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for NDRG1. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in NDRG1 reflect biology rather than handling. When interpreting NDRG1, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep NDRG1 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.