LOX Antibody

About the Target

Lysyl oxidase (LOX) is a secreted copper-dependent amine oxidase belonging to the LOX/LOXL family (which includes LOX and LOXL1-4) that catalyzes oxidative deamination of lysine and hydroxylysine residues in collagen and elastin and therefore plays a pivotal role in extracellular matrix cross-linking and stability. LOX is synthesized as a ~50 kDa pre-proenzyme containing an N-terminal signal peptide and pro-peptide region and a conserved C-terminal catalytic domain that harbors the lysyl-tyrosyl-quinone (LTQ) cofactor together with a copper-binding motif; the mature active enzyme (~30 kDa) is generated by proteolytic removal of the pro-region. Depending on the literature source, LOX may also be discussed as Protein-lysine 6-oxidase and Lysyl oxidase.

Reported cellular context includes secreted, which can matter when signal is compared across treatments or changing cell states. Following LOX across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

LOX is commonly interpreted in the context of cancer and cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans secreted, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• signal enrichment within secreted relative to the broader cellular background
• changes associated with proliferative state, oncogenic signaling, or treatment response
• signal-dependent shifts after ligand, inhibitor, or growth-factor perturbation
• co-patterning with orthogonal markers and control conditions that clarify pathway state

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for LOX. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in LOX reflect biology rather than handling. When interpreting LOX, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep LOX trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.