Phospho-LAT(Tyr200) Antibody

About the Target

Phospho-LAT (Tyr200) refers to the phosphorylated form of the adaptor protein LAT (Linker for Activation of T cells) at the tyrosine 200 residue (numbering varies between species; sometimes referred to as Tyr191 or Tyr226 in other contexts). LAT is a type III transmembrane protein with a short extracellular domain, a transmembrane region, and a cytosolic tail containing multiple conserved tyrosines that become phosphorylated upon T cell receptor (TCR) engagement. Depending on the literature source, LAT may also be discussed as Phospho-LAT(Tyr200) and Linker for activation of T-cells family member 1.

Reported cellular context includes cell membrane and membrane, which can matter when signal is compared across treatments or changing cell states. Following LAT across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

LAT is commonly interpreted in the context of immunology, developmental biology, and cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cell membrane and membrane, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between cell membrane and membrane across matched conditions
• context differences tied to immune-cell state, activation, or lineage composition
• stage-dependent patterns during differentiation, morphogenesis, or lineage commitment
• signal-dependent shifts after ligand, inhibitor, or growth-factor perturbation

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for LAT. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in LAT reflect biology rather than handling. When interpreting LAT, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep LAT trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.