KDM3A (JHDM2A) Antibody - Western Blot Validated
BiCell Scientific
SKU:82105-WB-50UL
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About the Target
This antibody supports experimental designs that evaluate KDM3A with interpretable controls and clear contrasts. KDM3A is a molecular target often examined to connect phenotype changes with underlying protein regulation. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.
In some datasets and protocols, it is referenced by the gene symbol JHDM2A.
Research Context
Comparative experiments across cell states can uncover patterns that single-condition measurements miss. Time course design can be decisive when regulation is transient or compartment dependent.
Consider these angles when interpreting target-level changes:
- baseline versus stimulated condition comparisons
- interpretation alongside orthogonal readouts
- cross-sample normalization strategies
Variant Considerations
When samples are prepared in reducing conditions, some epitopes become more accessible; this version is designed with that context in mind. This matters because epitope exposure and background can vary with preparation and extraction steps. Include controls that separate true target shifts from matrix or preparation effects.
For challenging targets, pairing KDM3A measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
If your study spans multiple model systems, it can help to define a shared reference condition for KDM3A so cross-sample contrasts stay meaningful.
Integrate the denaturing-format version into your design so KDM3A signals can be interpreted alongside matched controls.
- Targets:
- KDM3A
- Research Area:
- Epigenetics
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (15-aa) derived from the C-terminal region of mouse KDM3A protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9Y4C1
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 93.3% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C