ICAM1 (CD54) Antibody - FFPE Validated

About the Target

This antibody supports experimental designs that characterize ICAM1 with interpretable controls and clear contrasts. ICAM1 is a receptor-linked protein that can influence cell communication and downstream signaling. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

In some datasets and protocols, it is referenced by the gene symbol CD54.

Research Context

Because expression can be context dependent, matched controls and gating strategies help avoid over-interpretation. Time course design can be decisive when regulation is transient or compartment dependent.

Consider these angles when interpreting target-level changes:

• assessment of surface versus internalized pools
• comparisons across differentiation or polarization states
• panel-based interpretation with additional lineage markers

Variant Considerations

This fixed-section oriented version is intended for paraffin-embedded tissue, where crosslinking and retrieval steps can shape epitope accessibility. This matters because epitope exposure and background can vary with preparation and extraction steps. Include controls that separate true target shifts from matrix or preparation effects.

If your study spans multiple model systems, it can help to define a shared reference condition for ICAM1 so cross-sample contrasts stay meaningful.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so ICAM1 patterns can be compared across experiments without guesswork.

Use the fixed-section option to keep ICAM1 analysis aligned with planned contrasts and endpoints.