Histone H3 (di methyl Lys79) Antibody

About the Target

Di-Methyl-Histone H3 (Lys79), abbreviated as H3K79me2, refers to the addition of two methyl groups to lysine 79 of histone H3, a conserved modification catalyzed by the Dot1 family methyltransferases (Dot1 in yeast, DOT1L in mammals). Unlike many histone modifications found on the flexible N-terminal tails, lysine 79 is located within the structured globular domain of histone H3 and exposed on the nucleosome disk surface, allowing interaction with regulatory proteins. Depending on the literature source, H3 may also be discussed as Histone H3 (di methyl Lys79) and Dot1; Dot1L; H3; H3.1; H3K79; H3K79me2; Histone; histone modification; methyl histone.

Reported cellular context includes chromosome, nucleosome core, and nucleus, which can matter when signal is compared across treatments or changing cell states. Following H3 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

H3 is commonly interpreted in the context of epigenetics research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans chromosome, nucleosome core, and nucleus, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between chromosome, nucleosome core, and nucleus across matched conditions
• links between target behavior and transcriptional or chromatin-state changes
• differences between total target abundance and site-specific regulation when modified forms are compared
• co-patterning with orthogonal markers and control conditions that clarify pathway state

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for H3. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in H3 reflect biology rather than handling. When interpreting H3, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep H3 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.