GFAP Antibody - Western Blot Validated

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BiCell Scientific

SKU:02405-WB-50UL

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Size
Blocking Peptide
Quantity
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Scientific Overview

Build reproducible readouts by using this antibody to investigate GFAP across matched sample sets. GFAP is a target that supports characterization of cellular state through expression and localization readouts. For comparison-heavy studies, consistent sample handling and normalization help reduce run-to-run drift.

Tracking GFAP can clarify whether changes reflect regulation, redistribution, or altered complex assembly.

Research Relevance

Temporal sampling and matched controls can improve interpretation when expression is dynamic. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.

Because cellular networks are interconnected, changes in this target may coincide with shifts in related pathways, trafficking behavior, or structural remodeling. Neural differentiation and synaptic organization projects may include this target as a contextual readout.

When building a narrative around the target, it helps to track:

  • interpretation alongside orthogonal readouts
  • subcellular compartment mapping
  • cross-sample normalization strategies
  • baseline versus stimulated condition comparisons

Application-Specific Context

If your primary readout depends on denatured protein presentation, this option can streamline comparison across lanes and conditions. Think about how preparation affects epitope accessibility, and interpret patterns alongside morphology and compartment markers as needed. A short pilot series can help set exposure ranges and reduce reruns.

For challenging targets, pairing GFAP measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.

With consistent handling, the denaturing-format format can make GFAP comparisons easier to reproduce across runs.

Targets:
GFAP
Research Area:
Neuroscience
Application:
WB
Reactivity:
Human • Mouse • Rat
Host:
Rat
Clonality:
Polyclonal
Isotype:
IgG
Immunogen:
Synthetic peptide (18-aa) derived from the C-terminal region of human GFAP protein
Conjugation:
Unconjugated
Purification:
Affinity Chromatography
Concentration:
0.25 mg/ml
UniProt:
P14136
Homology:
Synthetic peptide sequence is identical to mouse or rat sequence
Storage Buffer:
PBS, pH 7.2, 0.1% Sodium Azide
Storage Temperature:
-20°C