Dual Luciferase Reporter Assay Kit

Overview

Dual Luciferase Reporter Assay Kit is a research-use kit supporting dual luciferase reporter assay workflows. Dual Luciferase Reporter Assay Kit can detect the expression of Luciferase regulated by gene elements sensitively and efficiently. Key notes from the supplied features include: Linear range up to 8 orders of magnitude (R²>0. Written for clarity and traceability, the description stays close to the supplied product information so your ordering and documentation remain consistent.

Key Benefits

• Suitable for the detection of weak promoters or low-expression regulationWide detection range: Linear range up to 8 orders of magnitude (R²>0.
• 99)High sensitivity: Detection sensitivity as low as 10-18 molComponents.
• Dual Luciferase Reporter Assay Kit can detect the expression of Luciferase regulated by gene elements sensitively and efficiently.
• Usually, the transcriptional regulatory element is cloned upstream of Firefly luciferase, or the 3'-UTR regulatory region is cloned downstream of firefly luciferase.
• The transfected cells are induced by corresponding stimulator and lysed to determine the luciferase activity.

How it supports your workflow

When you’re standardizing experiments across people, sites, or timepoints, consistent reagents and consistent documentation matter just as much as speed. For dual luciferase reporter assay workflows, the goal is often to keep the scientific rationale and the purchasing record aligned. To achieve dual luciferase reporter gene detection, Firefly luciferase is detected with Luciferin as a substrate, and Renilla Iuciferase is detected with coelenterazine as a substrate, while inhibiting the activity of Firefly luciferase. Dual Luciferase Reporter Assay Kit can detect the expression of Luciferase regulated by gene elements sensitively and efficiently. Usually, the transcriptional regulatory element is cloned upstream of Firefly luciferase, or the 3'-UTR regulatory region is cloned downstream of firefly luciferase. The supplied feature set highlights suitable for the detection of weak promoters or low-expression regulationwide detection range and 99)high sensitivity, which can help teams standardize language and expectations across similar products. Because projects evolve, we aim for descriptions that remain searchable, specific, and easy to compare across catalog numbers—so you can reorder confidently and keep documentation consistent.

Applications & compatibility

This section summarizes where the product fits based on the supplied description and features. Based on the supplied information, it’s positioned for dual luciferase reporter assay workflows where clear documentation and straightforward compatibility notes matter. If you’re matching this item to an established SOP, use the product name, catalog number, and the supplied features as the primary reference points. If your workflow spans multiple instruments or sample types, look for the compatibility wording here to keep product selection and documentation aligned.

Ideal for: groups that rely on dual luciferase reporter assay workflows and want a product listing that is specific, easy to reference, and careful about scope without introducing unsupported claims. Ideal when you need a quick, confident match between what’s on the page and what’s in your records.