Connexin-29 (Cx29 or Gjc3) Antibody
BiCell Scientific
SKU:00401-50UL
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Protein Function
When control selection matters, this antibody helps you analyze Connexin-29 (Cx29) in a structured study. Connexin-29 is a structural regulator of intercellular boundaries and tissue architecture. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.
In some datasets and protocols, it is referenced by the gene symbol Cx29.
Experimental Applications
In cultured models, junctional readouts can change rapidly after calcium modulation or cytoskeletal perturbation. Time course design can be decisive when regulation is transient or compartment dependent.
In practice, target tracking can support localization mapping, relative quantification, and interaction-focused experiments without locking you into a single readout strategy.
Common experimental questions that benefit from target tracking include:
- co-localization patterns with cytoskeletal elements
- subcellular redistribution at contact sites versus cytoplasm
- comparative profiling across cell states or perturbations
Workflow Perspective
For broad method development, the regular format can help you benchmark target behavior before narrowing to a specific readout style. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Predefine acceptance criteria for controls before collecting the full dataset.
For challenging targets, pairing Connexin-29 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
Integrate the regular format version into your design so Connexin-29 (Cx29) signals can be interpreted alongside matched controls.
- Targets:
- GJC3
- Research Area:
- Developmental Biology
- Application:
- IF • IHC • WB
- Reactivity:
- Mouse • Rat
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (17-aa) derived from the C-terminal region of mouse Connexin-29 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q8NFK1
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 52.9% homology to human sequence)Conjugate: Unconjugated
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C