Claudin-16 (CLDN16) (Phospho-Thr233) Antibody - Western Blot Validated

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BiCell Scientific

SKU:00216P-WB-50UL

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Blocking Peptide
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Protein Function

Bring Claudin-16 Phospho-Thr233 into view to examine changes across conditions. Claudin-16 Phospho-Thr233 is a junctional protein that coordinates adhesion with cytoskeletal anchoring. Focus on trends across conditions rather than single-point snapshots.

Monitoring Claudin-16 Phospho-Thr233 alongside context markers can improve interpretation when phenotypes are subtle.

Experimental Applications

Researchers often monitor junctional targets when studying epithelial integrity, remodeling, and motility. Pair measurements with orthogonal readouts when interpretation is sensitive to context.

Target-centric readouts can be framed around localization, relative abundance, or complex behavior, depending on the question you are testing.

Common experimental questions that benefit from target tracking include:

  • co-localization patterns with cytoskeletal elements
  • changes in complex assembly with scaffold or adaptor proteins
  • comparative profiling across cell states or perturbations
  • subcellular redistribution at contact sites versus cytoplasm

Workflow Perspective

When samples are prepared in reducing conditions, some epitopes become more accessible; this version is designed with that context in mind. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series.

If your study spans multiple model systems, it can help to define a shared reference condition for Claudin-16 Phospho-Thr233 so cross-sample contrasts stay meaningful.

For challenging targets, pairing Claudin-16 Phospho-Thr233 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.

Select the denaturing-format version to keep Claudin-16 Phospho-Thr233 interpretation aligned with your workflow goals.

Targets:
CLDN16
Research Area:
Developmental Biology
Application:
WB
Reactivity:
Human • Mouse • Rat
Host:
Rabbit
Clonality:
Polyclonal
Isotype:
IgG
Immunogen:
Synthetic peptide (11-aa) derived from the C-terminal region of mouse Claudin-16 protein with Thr233 modified by phosphorylation
Conjugation:
Unconjugated
Purification:
Affinity Chromatography
Concentration:
0.25 mg/ml
UniProt:
Q9Y5I7
Homology:
Synthetic peptide sequence is identical to rat sequence (Thr233) or human sequence (Thr303; human sequence has an additional 70-aa at the N-terminus)
Storage Buffer:
PBS, pH 7.2, 0.1% Sodium Azide
Storage Temperature:
-20°C