CEP85 (CCDC21) Antibody - FFPE Validated

Scientific Overview

Plan your comparisons around CEP85 and use this antibody to explore state-dependent differences. CEP85 is a microtubule-organizing element often evaluated in cell-cycle and cilia studies. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.

In some datasets and protocols, it is referenced by the gene symbol CCDC21.

Research Relevance

Quantitative comparisons often rely on consistent imaging parameters and clear inclusion criteria for ciliated cells. Time course design can be decisive when regulation is transient or compartment dependent.

When cell-state transitions occur, this target may change alongside partners in the same compartment or pathway neighborhood.

When building a narrative around the target, it helps to track:

• co-occurrence with intraflagellar transport markers
• signal enrichment at basal bodies versus cytoplasm
• changes in cilia incidence or apparent length in perturbation studies

Application-Specific Context

In sectioned tissue workflows, interpretation often depends on morphology; this format supports that style of readout. Think about how preparation affects epitope accessibility, and interpret patterns alongside morphology and compartment markers as needed. Align controls to the expected direction of change to support confident interpretation.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so CEP85 patterns can be compared across experiments without guesswork.

The fixed-section format fits designs where CEP85 is evaluated with deliberate controls and careful comparisons.