CEP85 (CCDC21) Antibody
BiCell Scientific
SKU:01078-50UL
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Protein Function
This antibody helps you evaluate CEP85 (CCDC21) while keeping condition-to-condition contrasts straightforward. CEP85 is a microtubule-organizing element often evaluated in cell-cycle and cilia studies. Use matched handling so differences reflect biology rather than processing.
In some datasets and protocols, it is referenced by the gene symbol CCDC21.
Experimental Applications
Ciliary proteins are frequently assessed in relation to cell-cycle stage, serum conditions, and differentiation cues. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
Many workflows benefit from tracking both signal intensity and compartment distribution, especially when the target participates in multi-protein assemblies.
Common experimental questions that benefit from target tracking include:
- co-occurrence with intraflagellar transport markers
- cell-cycle linked differences in localization
- signal enrichment at basal bodies versus cytoplasm
- changes in cilia incidence or apparent length in perturbation studies
Workflow Perspective
For broad method development, the regular format can help you benchmark target behavior before narrowing to a specific readout style. To keep comparisons consistent, align sample preparation steps and define a clear baseline for each series. Document normalization choices so collaborators can reproduce the same interpretation.
For challenging targets, pairing CEP85 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
Pair this regular format format with stable controls to keep CEP85 (CCDC21) measurements coherent over time.
- Targets:
- CEP85
- Research Area:
- Cell Cycle • Developmental Biology
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (18-aa) derived from the N-terminal region of mouse CEP85 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q6P2H3
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 77.8% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C