CEP250 Antibody - Western Blot Validated
BiCell Scientific
SKU:01095-WB-50UL
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Scientific Overview
Designed to help you explore CEP250, this antibody supports studies where protein context matters. CEP250 is a microtubule-organizing element often evaluated in cell-cycle and cilia studies. Its behavior can reflect changes in differentiation state, stress, or remodeling of cellular architecture.
Monitoring CEP250 alongside context markers can improve interpretation when phenotypes are subtle.
Research Relevance
Disruption of intraflagellar transport or basal body function can produce distinct localization signatures. Because regulation can be multi-layered, combining abundance and localization information can sharpen conclusions.
Since many cellular processes are coupled, measured differences can reflect both direct regulation and indirect effects from broader state changes.
When building a narrative around the target, it helps to track:
- changes in cilia incidence or apparent length in perturbation studies
- cell-cycle linked differences in localization
- co-occurrence with intraflagellar transport markers
Application-Specific Context
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. Think about how preparation affects epitope accessibility, and interpret patterns alongside morphology and compartment markers as needed.
When interpreting CEP250, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
When interpreting CEP250, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
Use the denaturing-format variant when your project demands CEP250 readouts that respect preparation constraints.
- Targets:
- CEP250
- Research Area:
- Cell Cycle • Developmental Biology
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (16-aa) derived from the N-terminal region of mouse CEP250 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9BV73
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 87.5% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C