CEP164 (NPHP15) Antibody - Western Blot Validated

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BiCell Scientific

SKU:01092-WB-50UL

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Size
Blocking Peptide
Quantity
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About the Target

To connect mechanism with measurement, this antibody lets you analyze CEP164 (NPHP15) in pathway-driven studies. CEP164 is a microtubule-organizing element often evaluated in cell-cycle and cilia studies. Many projects interpret this target alongside interacting partners, upstream cues, and downstream readouts.

In some datasets and protocols, it is referenced by the gene symbol NPHP15.

Research Context

Ciliary proteins are frequently assessed in relation to cell-cycle stage, serum conditions, and differentiation cues. Considering complex membership and compartment shifts can clarify whether changes reflect signaling or redistribution.

Consider these angles when interpreting target-level changes:

  • signal enrichment at basal bodies versus cytoplasm
  • cell-cycle linked differences in localization
  • changes in cilia incidence or apparent length in perturbation studies

Variant Considerations

This denaturing-format version is geared toward targets presented after reduction and denaturation, where epitope exposure can shift from native conformations. This matters because epitope exposure and background can vary with preparation and extraction steps. If you compare conditions, keep processing steps uniform to support clean interpretation.

For challenging targets, pairing CEP164 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.

To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so CEP164 patterns can be compared across experiments without guesswork.

The denaturing-format option supports mechanistic reading of CEP164 (NPHP15) when interactions and context are the focus.

Targets:
CEP164
Research Area:
Cell Cycle • Developmental Biology
Application:
WB
Reactivity:
Human • Mouse • Rat
Host:
Rabbit
Clonality:
Polyclonal
Isotype:
IgG
Immunogen:
Synthetic peptide (15-aa) derived from the N-terminal region of mouse CEP164 protein
Conjugation:
Unconjugated
Purification:
Affinity Chromatography
Concentration:
0.25 mg/ml
UniProt:
Q9UPV0
Homology:
Synthetic peptide sequence is identical to rat sequence (showing 66.7% homology to human sequence)
Storage Buffer:
PBS, pH 7.2, 0.1% Sodium Azide
Storage Temperature:
-20°C