CEP131 Antibody
BiCell Scientific
SKU:01086-50UL
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About the Target
This antibody supports experimental designs that explore CEP131 with interpretable controls and clear contrasts. CEP131 is a microtubule-organizing element often evaluated in cell-cycle and cilia studies. Plan positive and negative controls that match your model system, including perturbations expected to shift the target.
Following CEP131 across perturbations can help separate expression effects from compartment shifts.
Research Context
Ciliary proteins are frequently assessed in relation to cell-cycle stage, serum conditions, and differentiation cues. Time course design can be decisive when regulation is transient or compartment dependent.
Consider these angles when interpreting target-level changes:
- co-occurrence with intraflagellar transport markers
- changes in cilia incidence or apparent length in perturbation studies
- signal enrichment at basal bodies versus cytoplasm
Variant Considerations
For broad method development, the regular format can help you benchmark target behavior before narrowing to a specific readout style. This matters because epitope exposure and background can vary with preparation and extraction steps. Include controls that separate true target shifts from matrix or preparation effects.
To support downstream analysis, record key context variables (culture timing, stimulation window, and extraction approach) so CEP131 patterns can be compared across experiments without guesswork.
When interpreting CEP131, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
The regular format format fits designs where CEP131 is evaluated with deliberate controls and careful comparisons.
- Targets:
- CEP131
- Research Area:
- Cell Cycle • Developmental Biology
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (15-aa) derived from the N-terminal region of mouse CEP131 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q9UPN4
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 66.7% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C