Centrin-1 Antibody
BiCell Scientific
SKU:01061-50UL
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Scientific Overview
For consistent comparison across experiments, use this antibody to explore Centrin-1 with a repeatable workflow. Centrin-1 is a ciliary or centrosomal protein connected to microtubule-based organization. Use matched handling so differences reflect biology rather than processing.
Monitoring Centrin-1 alongside context markers can improve interpretation when phenotypes are subtle.
Research Relevance
Quantitative comparisons often rely on consistent imaging parameters and clear inclusion criteria for ciliated cells. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
When cell-state transitions occur, this target may change alongside partners in the same compartment or pathway neighborhood.
When building a narrative around the target, it helps to track:
- cell-cycle linked differences in localization
- changes in cilia incidence or apparent length in perturbation studies
- signal enrichment at basal bodies versus cytoplasm
- co-occurrence with intraflagellar transport markers
Application-Specific Context
When assay conditions are still being tuned, a regular-format antibody supports stepwise adjustment of buffers and incubation steps. Think about how preparation affects epitope accessibility, and interpret patterns alongside morphology and compartment markers as needed. A short pilot series can help set exposure ranges and reduce reruns.
If your study spans multiple model systems, it can help to define a shared reference condition for Centrin-1 so cross-sample contrasts stay meaningful.
Pair this regular format format with stable controls to keep Centrin-1 measurements coherent over time.
- Targets:
- CETN1
- Research Area:
- Cell Cycle • Developmental Biology
- Application:
- IF • IHC • WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (18-aa) derived from the C-terminal region of mouse Centrin-1 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q12798
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 94.4% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C