CD97 Antibody

About the Target

CD97, a member of the adhesion GPCR family, integrates cell adhesion and signalling through its extracellular EGF-like domains and seven transmembrane structure. Its isoforms, EGF(1-5) and EGF(1,2,5) mediate distinct functions: EGF(1-5) interacts with chondroitin sulfate and integrins (α5β1) to drive tumor invasion and angiogenesis, while EGF(1,2,5) binds CD55, regulating immune cell migration and T-cell activation.

Reported cellular context includes cell membrane, membrane, and secreted, which can matter when signal is compared across treatments or changing cell states. Following CD97 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.

Research Context

CD97 is commonly interpreted in the context of cancer and immunology research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cell membrane, membrane, and secreted, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.

Consider these angles when interpreting target-level changes:

• apparent redistribution between cell membrane, membrane, and secreted across matched conditions
• changes associated with proliferative state, oncogenic signaling, or treatment response
• context differences tied to immune-cell state, activation, or lineage composition
• co-patterning with orthogonal markers and control conditions that clarify pathway state

Variant Considerations

If your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for CD97. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.

Standardize sampling time, control choice, and downstream analysis thresholds so apparent differences in CD97 reflect biology rather than handling. When interpreting CD97, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.

For multi-run studies, a shared reference condition can keep CD97 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.