BAX (BCL2L4) Antibody - Western Blot Validated
BiCell Scientific
SKU:10254-WB-50UL
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Scientific Overview
Bring BAX (BCL2L4) into view to explore changes across conditions. BAX is a protein of interest for mapping abundance changes across conditions and cell states. Look for shifts in distribution that track with phenotype changes.
Following BAX across perturbations can help separate expression effects from compartment shifts.
Research Relevance
In many projects, target-level measurements help link perturbations to downstream phenotypes. Pair measurements with orthogonal readouts when interpretation is sensitive to context.
When cell-state transitions occur, this target may change alongside partners in the same compartment or pathway neighborhood.
When building a narrative around the target, it helps to track:
- interpretation alongside orthogonal readouts
- subcellular compartment mapping
- cross-sample normalization strategies
- baseline versus stimulated condition comparisons
Application-Specific Context
For size-resolved readouts under denaturing conditions, this version helps you focus on expected band patterns and molecular weight regions. Think about how preparation affects epitope accessibility, and interpret patterns alongside morphology and compartment markers as needed.
When interpreting BAX, consider whether changes track with compartment identity, cell density, or perturbation timing; these factors can influence apparent signal without implying a direct causal link.
For challenging targets, pairing BAX measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
Choose the denaturing-format option when you want BAX (BCL2L4) results aligned to your sample format.
- Targets:
- BAX
- Research Area:
- Apoptosis • Metabolism
- Application:
- WB
- Reactivity:
- Human • Mouse • Rat
- Host:
- Rat
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (15-aa) derived from the N-terminal region of mouse BAX protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- Q07812
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 80% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C