Aquaporin-7 (AQP7) Antibody - Western Blot Validated
BiCell Scientific
SKU:20107-WB-50UL
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About the Target
Build reproducible readouts by using this antibody to investigate Aquaporin-7 across matched sample sets. Aquaporin-7 is a membrane-embedded regulator of ionic or osmotic balance in cells. For comparison-heavy studies, consistent sample handling and normalization help reduce run-to-run drift.
In some datasets and protocols, it is referenced by the gene symbol AQP7.
Research Context
Many transport proteins cycle through endosomal pathways, making localization a key readout alongside abundance. Define inclusion criteria and controls early so replicate sets remain interpretable as the dataset grows.
Consider these angles when interpreting target-level changes:
- patterns consistent with apical or basolateral sorting
- stimulus-dependent trafficking and turnover
- relative abundance across engineered constructs
- surface versus intracellular pool distribution
Variant Considerations
This denaturing-format version is geared toward targets presented after reduction and denaturation, where epitope exposure can shift from native conformations. This matters because epitope exposure and background can vary with preparation and extraction steps. Standardize key parameters so differences reflect biology rather than handling.
For challenging targets, pairing Aquaporin-7 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
For challenging targets, pairing Aquaporin-7 measurements with a second marker can clarify whether differences arise from regulation, redistribution, or shifts in cell composition within the sample.
Pair this denaturing-format format with stable controls to keep Aquaporin-7 measurements coherent over time.
- Targets:
- AQP7
- Research Area:
- Metabolism
- Application:
- WB
- Reactivity:
- Mouse • Rat
- Host:
- Rabbit
- Clonality:
- Polyclonal
- Isotype:
- IgG
- Immunogen:
- Synthetic peptide (18-aa) derived from the C-terminal region of mouse Aquaporin-7 protein
- Conjugation:
- Unconjugated
- Purification:
- Affinity Chromatography
- Concentration:
- 0.25 mg/ml
- UniProt:
- O14520
- Homology:
- Synthetic peptide sequence is identical to rat sequence (showing 38.9% homology to human sequence)
- Storage Buffer:
- PBS, pH 7.2, 0.1% Sodium Azide
- Storage Temperature:
- -20°C