{"product_id":"vps35-antibody-sc-f2969","title":"VPS35 Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003eVPS35 (Vacuolar Protein Sorting 35) is a core component of the retromer complex, essential for the retrograde transport of transmembrane proteins from endosomes to the trans-Golgi network (TGN) and plasma membrane. It is a 796-amino acid protein with an α-helical solenoid structure, where its N-terminal region binds VPS26 and the C-terminal region interacts with VPS29 to stabilize the retromer complex.\u003c\/p\u003e\u003cp\u003eReported cellular context includes cytoplasm, endosome, and membrane, which can matter when signal is compared across treatments or changing cell states. Following VPS35 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003eVPS35 is commonly interpreted in the context of neuroscience and metabolism research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cytoplasm, endosome, and membrane, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eapparent redistribution between cytoplasm, endosome, and membrane across matched conditions\u003c\/li\u003e\n\u003cli\u003ecompartment-specific patterns relevant to neuronal polarity, transport, or synaptic context\u003c\/li\u003e\n\u003cli\u003eresponses linked to nutrient status, mitochondrial state, or metabolic rewiring\u003c\/li\u003e\n\u003cli\u003eco-patterning with orthogonal markers and control conditions that clarify pathway state\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for VPS35. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in VPS35 reflect biology rather than handling. When interpreting VPS35, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep VPS35 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57577988620633,"sku":"F2969-20UL","price":149.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57577988653401,"sku":"F2969-100UL","price":329.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57577988686169,"sku":"F2969-2X100UL","price":489.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F2969-IF.png?v=1773600850","url":"https:\/\/absource-diagnostics.myshopify.com\/products\/vps35-antibody-sc-f2969","provider":"Absource Diagnostics","version":"1.0","type":"link"}