{"product_id":"vav1-antibody-sc-f2560","title":"Phospho-VAV1 (Tyr174) Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003ePhospho-VAV1 (Tyr174) refers to the phosphorylated form of the Vav1 protein at tyrosine residue 174, a critical regulatory site within its acidic (Ac) region. Structurally, Vav1 is a multi-domain guanine nucleotide exchange factor (GEF) containing a Dbl homology (DH) domain essential for activating Rho family GTPases, flanked by a pleckstrin homology (PH) domain, with additional calponin homology (CH), C1, SH2, and SH3 domains. Depending on the literature source, VAV1 may also be discussed as Phospho-VAV1 (Tyr174) and VAV.\u003c\/p\u003e\u003cp\u003eReported cellular context includes cell-cell junction, cytoplasm, cytosol, and plasma membrane, which can matter when signal is compared across treatments or changing cell states. Following VAV1 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003eVAV1 is commonly interpreted in the context of cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cell-cell junction, cytoplasm, and cytosol, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eapparent redistribution between cell-cell junction, cytoplasm, and cytosol across matched conditions\u003c\/li\u003e\n\u003cli\u003esignal-dependent shifts after ligand, inhibitor, or growth-factor perturbation\u003c\/li\u003e\n\u003cli\u003edifferences between total target abundance and site-specific regulation when modified forms are compared\u003c\/li\u003e\n\u003cli\u003eco-patterning with orthogonal markers and control conditions that clarify pathway state\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for VAV1. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in VAV1 reflect biology rather than handling. When interpreting VAV1, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep VAV1 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57577959981401,"sku":"F2560-20UL","price":199.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57577960014169,"sku":"F2560-100UL","price":489.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57577960046937,"sku":"F2560-2X100UL","price":729.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F2560-IF.png?v=1773600535","url":"https:\/\/absource-diagnostics.myshopify.com\/products\/vav1-antibody-sc-f2560","provider":"Absource Diagnostics","version":"1.0","type":"link"}