{"product_id":"prkdc-antibody-sc-f1129","title":"DNA PKcs Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003ePRKDC is a target of interest in many antibody-based workflows. DNA-dependent protein kinase catalytic subunit (DNA-PKcs) belongs to the phosphatidylinositol 3-kinase-related kinase family and is a critical component of the DNA damage response (DDR). It forms the DNA-PK holoenzyme along with the Ku80\/Ku70 heterodimer. Upon DNA double-strand breaks (DSBs), DNA-PKcs are promptly recruited to the damaged sites and is activated through auto-phosphorylation and phosphorylation by Ataxia-Telangiectasia Mutated (ATM). Depending on the literature source, PRKDC may also be discussed as DNA PKcs and DNA-PK.\u003c\/p\u003e\u003cp\u003eReported cellular context includes nucleus, which can matter when signal is compared across treatments or changing cell states. Following PRKDC across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003ePRKDC is commonly interpreted in the context of cancer and dna damage \/ repair research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans nucleus, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003esignal enrichment within nucleus relative to the broader cellular background\u003c\/li\u003e\n\u003cli\u003echanges associated with proliferative state, oncogenic signaling, or treatment response\u003c\/li\u003e\n\u003cli\u003estress-induced changes after checkpoint activation or genotoxic challenge\u003c\/li\u003e\n\u003cli\u003eco-patterning with orthogonal markers and control conditions that clarify pathway state\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for PRKDC. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in PRKDC reflect biology rather than handling. When interpreting PRKDC, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep PRKDC trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57577619587417,"sku":"F1129-20UL","price":159.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57577619620185,"sku":"F1129-100UL","price":339.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57577619652953,"sku":"F1129-2X100UL","price":499.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F1129-IHC1.jpg?v=1773599320","url":"https:\/\/absource-diagnostics.myshopify.com\/products\/prkdc-antibody-sc-f1129","provider":"Absource Diagnostics","version":"1.0","type":"link"}