{"product_id":"p-rela-antibody-sc-f0155","title":"Phospho-NF-κB p65 (Ser536) Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003eP-RELA is a target of interest in many antibody-based workflows. NF-κB is activated by various infectious agents and inflammatory cytokines, playing a central role in inflammation control and immunosuppression. The mammalian NF-κB family comprises five transcription factors: p65 (RelA), RelB, c-Rel, p105\/p50 (NF-κB1), and p100\/p52 (NF-κB2), with p50 and p52 generated by proteolytic processing of p105 and p100. Depending on the literature source, P-RELA may also be discussed as Phospho-NF-kappaB p65 (Ser536) and NF-kB p65 (phospho S536).\u003c\/p\u003e\u003cp\u003eReported cellular context includes cytoplasm and nucleus, which can matter when signal is compared across treatments or changing cell states. Following P-RELA across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003eP-RELA is commonly interpreted in the context of immunology and inflammation research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cytoplasm and nucleus, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eapparent redistribution between cytoplasm and nucleus across matched conditions\u003c\/li\u003e\n\u003cli\u003econtext differences tied to immune-cell state, activation, or lineage composition\u003c\/li\u003e\n\u003cli\u003eresponses associated with cytokine exposure, inflammatory tone, or tissue stress\u003c\/li\u003e\n\u003cli\u003edifferences between total target abundance and site-specific regulation when modified forms are compared\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for P-RELA. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in P-RELA reflect biology rather than handling. When interpreting P-RELA, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep P-RELA trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57577385394521,"sku":"F0155-20UL","price":159.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57577385427289,"sku":"F0155-100UL","price":389.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57577385460057,"sku":"F0155-2X100UL","price":579.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F0155-IF.png?v=1773598157","url":"https:\/\/absource-diagnostics.myshopify.com\/products\/p-rela-antibody-sc-f0155","provider":"Absource Diagnostics","version":"1.0","type":"link"}