{"product_id":"grasp65-antibody-sc-f3333","title":"GRASP65 C-terminal Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003eGRASP65 is a peripheral Golgi matrix protein featuring an N-terminal region with tandem PDZ domains that mediate oligomerization and membrane tethering and a C-terminal serine\/proline-rich domain dynamically regulated by phosphorylation. This C-terminal region acts as a key regulatory hub, where mitotic kinases such as Cdk1 and Plk1 phosphorylate specific serine and threonine residues (e. g., T220\/T224, S277, S376), triggering disassembly of GRASP65 oligomers and leading to Golgi cisternal unstacking and fragmentation during mitosis. Depending on the literature source, GRASP65 may also be discussed as GRASP65 C-terminal and GOLPH5.\u003c\/p\u003e\u003cp\u003eReported cellular context includes golgiapparatus and membrane, which can matter when signal is compared across treatments or changing cell states. Following GRASP65 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003eGRASP65 is commonly interpreted in the context of cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans golgiapparatus and membrane, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eapparent redistribution between golgiapparatus and membrane across matched conditions\u003c\/li\u003e\n\u003cli\u003esignal-dependent shifts after ligand, inhibitor, or growth-factor perturbation\u003c\/li\u003e\n\u003cli\u003eco-patterning with orthogonal markers and control conditions that clarify pathway state\u003c\/li\u003e\n\u003cli\u003etime-matched comparisons so changes reflect biology rather than handling or sampling drift\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for GRASP65. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in GRASP65 reflect biology rather than handling. When interpreting GRASP65, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep GRASP65 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57578015654233,"sku":"F3333-20UL","price":199.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57578015687001,"sku":"F3333-100UL","price":489.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57578015719769,"sku":"F3333-2X100UL","price":729.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F3333-IF.png?v=1773601122","url":"https:\/\/absource-diagnostics.myshopify.com\/products\/grasp65-antibody-sc-f3333","provider":"Absource Diagnostics","version":"1.0","type":"link"}