{"product_id":"bub3-antibody-sc-f2994","title":"Bub3 Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003eBub3 (Budding Uninhibited by Benzimidazole 3) is a conserved mitotic checkpoint protein essential for the Spindle Assembly Checkpoint (SAC), ensuring accurate chromosome segregation during mitosis and meiosis by preventing premature anaphase onset. Structurally, Bub3 is a WD40-repeat protein with a seven-blade β-propeller conformation that facilitates protein-protein interactions, particularly with SAC components like Bub1 and BubR1 via their Gle2-binding sequence (GLEBS) motifs.\u003c\/p\u003e\u003cp\u003eReported cellular context includes centromere, chromosome, kinetochore, and nucleus, which can matter when signal is compared across treatments or changing cell states. Following BUB3 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003eBUB3 is commonly interpreted in the context of cell cycle research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans centromere, chromosome, and kinetochore, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eapparent redistribution between centromere, chromosome, and kinetochore across matched conditions\u003c\/li\u003e\n\u003cli\u003ecell-cycle linked differences in abundance, timing, or compartmental enrichment\u003c\/li\u003e\n\u003cli\u003eco-patterning with orthogonal markers and control conditions that clarify pathway state\u003c\/li\u003e\n\u003cli\u003etime-matched comparisons so changes reflect biology rather than handling or sampling drift\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for BUB3. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in BUB3 reflect biology rather than handling. When interpreting BUB3, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep BUB3 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57577990324569,"sku":"F2994-20UL","price":149.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57577990357337,"sku":"F2994-100UL","price":329.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57577990390105,"sku":"F2994-2X100UL","price":489.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F2994-IF.png?v=1773600872","url":"https:\/\/absource-diagnostics.myshopify.com\/products\/bub3-antibody-sc-f2994","provider":"Absource Diagnostics","version":"1.0","type":"link"}