{"product_id":"box5-p1216","title":"Box5","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003eBox5, a Wnt5a-derived N-butyloxycarbonyl hexapeptide (t-Boc-Met-Asp-Gly-Cys-Glu-Leu), acts as an Wnt5a antagonist, inhibits the basal migration and invasion of Wnt5a-expressing HTB63 melanoma cells, antagonizes the effects of Wnt5a on melanoma cell migration and invasion by directly inhibiting Wnt5a-induced PKC and Ca (2+) signaling. The mapped target for this entry is Protein Wnt-5a (WNT5A). This target context is most often investigated as part of ligand-responsive signaling, where receptor occupancy can reshape downstream second-messenger output, trafficking, secretion, excitability, or transcriptional programs. Across mechanistic studies, investigators commonly track acute pathway activation, receptor trafficking, and downstream transcriptional changes. In practical terms, this makes the product most relevant to experiments that need a defined and reversible way to perturb biology over short time scales.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003eAs an antagonist-format peptide, it is well suited to experiments that test pathway dependence, receptor blockade, and the extent to which a phenotype requires ongoing target signaling. In practice, dose-response design, timing, and matched control conditions are important for separating direct target engagement from delayed compensatory responses.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003epair peptide treatment with pathway-proximal signaling or trafficking readouts whenever possible\u003c\/li\u003e\n\u003cli\u003ecompare responses across cell states or model systems with different receptor abundance\u003c\/li\u003e\n\u003cli\u003edistinguish primary target engagement from downstream adaptation during longer incubations\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eExperimental interpretation should therefore connect early pathway changes with later phenotypic outputs, rather than relying on a single endpoint in isolation.\u003c\/p\u003e\u003ch2\u003eFormat Considerations\u003c\/h2\u003e\u003cp\u003eFor routine mechanistic work, the unmodified catalog format provides a consistent starting point for concentration-response studies, benchmark experiments, and orthogonal validation. In comparative workflows, consistency of preparation, exposure window, and matched controls is often as important as the nominal treatment itself. This is particularly helpful for comparative experiments, benchmark studies, and orthogonal validation in which small differences in formulation or handling can complicate interpretation. For peptide-centered workflows, conclusions are usually strongest when biological readouts are paired with consistent preparation and appropriately matched reference conditions.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"5 mg","offer_id":57636816126297,"sku":"P1216-5MG","price":186.0,"currency_code":"EUR","in_stock":true},{"title":"25 mg","offer_id":57636816159065,"sku":"P1216-25MG","price":560.0,"currency_code":"EUR","in_stock":true},{"title":"100 mg","offer_id":57636816191833,"sku":"P1216-100MG","price":1402.0,"currency_code":"EUR","in_stock":true},{"title":"1 g","offer_id":57636816224601,"sku":"P1216-1G","price":4904.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/p1216-box5-chemical-structure.gif?v=1774212253","url":"https:\/\/absource-diagnostics.myshopify.com\/products\/box5-p1216","provider":"Absource Diagnostics","version":"1.0","type":"link"}