{"product_id":"appl1-antibody-sc-f0618","title":"APPL1 Antibody","description":"\u003ch2\u003eAbout the Target\u003c\/h2\u003e\u003cp\u003eAPPL1 is an Akt\/protein kinase B-binding protein involved in signal transduction and membrane trafficking pathways. In humans, APPL1 is highly expressed in different tissues such as the pancreas, liver, adipose tissue, brain, and muscles. APPL1 is a 710 amino acid protein constituted of 5 different functional domains: one NH2-terminal Bin\/Amphiphysin\/Rvs domain (BAR), a central pleckstrin homology domain (PH), a BPP domain, a COOH-terminal phosphotyrosine-binding domain (PTB) and a coiled-coil (CC domain) through which it interacts with a large number of proteins including critical components of the insulin-signaling pathway. Depending on the literature source, APPL1 may also be discussed as APPL.\u003c\/p\u003e\u003cp\u003eReported cellular context includes cell projection, cytoplasm, cytoplasmic vesicle, and endosome, which can matter when signal is compared across treatments or changing cell states. Following APPL1 across matched perturbations can help separate abundance effects from shifts in localization, complex assembly, or pathway state.\u003c\/p\u003e\u003ch2\u003eResearch Context\u003c\/h2\u003e\u003cp\u003eAPPL1 is commonly interpreted in the context of cell signaling research, and readouts are often stronger when a study separates expression changes from compartment-level redistribution. When reported signal spans cell projection, cytoplasm, and cytoplasmic vesicle, a defined reference condition can make comparisons more interpretable across perturbations, passages, or replicate sets.\u003c\/p\u003e\u003cp\u003eConsider these angles when interpreting target-level changes:\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003eapparent redistribution between cell projection, cytoplasm, and cytoplasmic vesicle across matched conditions\u003c\/li\u003e\n\u003cli\u003esignal-dependent shifts after ligand, inhibitor, or growth-factor perturbation\u003c\/li\u003e\n\u003cli\u003eco-patterning with orthogonal markers and control conditions that clarify pathway state\u003c\/li\u003e\n\u003cli\u003etime-matched comparisons so changes reflect biology rather than handling or sampling drift\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eVariant Considerations\u003c\/h2\u003e\u003cp\u003eIf your project spans exploratory questions, the regular version offers a balanced option for establishing baseline signal behavior for APPL1. This can help when protocols evolve over time and the goal is to compare experiments using a stable reference workflow.\u003c\/p\u003e\u003cp\u003eStandardize sampling time, control choice, and downstream analysis thresholds so apparent differences in APPL1 reflect biology rather than handling. When interpreting APPL1, it is often useful to decide early whether the main question is overall abundance, compartmental enrichment, or context-dependent redistribution.\u003c\/p\u003e\u003cp\u003eFor multi-run studies, a shared reference condition can keep APPL1 trends easier to compare across datasets. That kind of consistency is especially helpful when follow-up work expands to new perturbations, model systems, or longitudinal collections.\u003c\/p\u003e","brand":"Selleck Chemicals","offers":[{"title":"20 µl","offer_id":57577491857753,"sku":"F0618-20UL","price":149.0,"currency_code":"EUR","in_stock":true},{"title":"100 µl","offer_id":57577491890521,"sku":"F0618-100UL","price":359.0,"currency_code":"EUR","in_stock":true},{"title":"2 × 100 µl","offer_id":57577491923289,"sku":"F0618-2X100UL","price":539.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/F0618-IF.png?v=1773598701","url":"https:\/\/absource-diagnostics.myshopify.com\/products\/appl1-antibody-sc-f0618","provider":"Absource Diagnostics","version":"1.0","type":"link"}