{"product_id":"affinity-maturation-or-degeneration-for-custom-antibody","title":"Affinity Maturation or Degeneration for Custom Antibody","description":"\u003cp\u003eThe \u003cstrong\u003eAffinity Maturation or Degeneration for Custom Antibody\u003c\/strong\u003e service from \u003cstrong\u003eBiCell Scientific\u003c\/strong\u003e is designed to experimentally modify the binding affinity of custom-made or existing monoclonal antibodies. The service can be used to increase antibody affinity through maturation or reduce binding affinity through degeneration, depending on the intended research application.\u003c\/p\u003e\n\u003cp\u003eBiCell Scientific uses proprietary \u003cstrong\u003eyeast 2-hybrid\u003c\/strong\u003e and \u003cstrong\u003eyeast display\u003c\/strong\u003e platforms to screen mutations in the complementarity-determining regions (CDRs) of the antibody variable domains. This approach supports antibody optimization for applications such as antibody repurposing, humanization, safety improvement and bispecific antibody binding adjustment.\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCustom affinity maturation or degeneration of monoclonal antibodies\u003c\/li\u003e\n\u003cli\u003eDesigned for custom-made or existing therapeutic monoclonal antibodies\u003c\/li\u003e\n\u003cli\u003eUses yeast 2-hybrid and yeast display screening platforms\u003c\/li\u003e\n\u003cli\u003eTargets mutations in CDR regions of heavy and light chain Fv domains\u003c\/li\u003e\n\u003cli\u003eSuitable for full-length protein, extracellular protein or peptide antigen projects\u003c\/li\u003e\n\u003cli\u003eCan support antibody repurposing, humanization and bispecific antibody optimization\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eService Workflow\u003c\/h2\u003e\n\u003cp\u003eThe workflow begins with cloning the scFv domain from the customer’s antibody into a yeast display vector. Random mutations are introduced into the CDR1–3 regions using error-prone PCR. The resulting antibody library is screened using MACS and FACS sorting to enrich antibody clones with the desired binding profile.\u003c\/p\u003e\n\u003cp\u003eFollowing clonal selection, selected antibody sequences can be cloned into expression vectors and expressed in HEK293 or CHO cells. The project deliverables may include sequenced Fv domains, expression vectors and purified engineered monoclonal antibody.\u003c\/p\u003e\n\u003ch2\u003eTypical Deliverables\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFully sequenced Fv domains of the engineered antibody heavy and light chains\u003c\/li\u003e\n\u003cli\u003eExpression vectors for the selected engineered antibody\u003c\/li\u003e\n\u003cli\u003e1 mg of affinity-purified engineered monoclonal antibody\u003c\/li\u003e\n\u003cli\u003eFACS-based screening data, depending on project scope\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eResearch Applications\u003c\/h2\u003e\n\u003cp\u003eThis service is suitable for research groups working on monoclonal antibody engineering, antibody discovery, therapeutic antibody repurposing, antibody humanization or bispecific antibody development. Affinity maturation may help improve antigen binding, while affinity degeneration can be useful when reduced binding strength is desired for a specific experimental design.\u003c\/p\u003e","brand":"BiCell Scientific","offers":[{"title":"1 mg","offer_id":58120496087385,"sku":"BCHUAD","price":12950.0,"currency_code":"EUR","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0923\/1011\/0553\/files\/affinitymuaturationimage2.jpg?v=1779460755","url":"https:\/\/absource-diagnostics.myshopify.com\/products\/affinity-maturation-or-degeneration-for-custom-antibody","provider":"Absource Diagnostics","version":"1.0","type":"link"}